TLC Image Annotation Tool
Overview
The TLC Image Annotation Tool is a lightweight, web-based application designed for annotating Thin Layer Chromatography (TLC) plates, calculating Rf values, and exporting annotated results. It can be used as a standalone tool or integrated into the Chemotion.
This tool supports the digitization of TLC documentation, automates Rf value calculations, and promotes consistent and well-structured ELN records.
Purpose
The tool bridges manual TLC processing with structured digital workflows.
- Improve accuracy in RF calculations
- Add clear, editable annotations to TLC images
- Save and export results for reproducibility and sharing
Features
- Flexible Image Input
- Upload your own (local) images
- Load TLC images directly from the Chemotion
- Mark reference lines, sample spots, and separation lanes
- Automatically calculate Rf values
- Versatile Output Options
- Export to CSV or annotated PNG
- Push results back to the Chemotion seamlessly
Supported Input
| Feature | Details |
|---|---|
| Image Types | JPG, PNG |
| Max File Size | 10 MB |
| Image Sources | Chemotion, Local Upload |
| Output Formats | CSV, Annotated PNG, Chemotion Upload |
Example Workflow
- Load an Image (from Chemotion or local)
- Draw Reference Lines (Min/Max)
- Mark Spots for your compounds
- Add Optional Labels (lane separators)
- Click ‘Calculate RF’ to compute values
- Export or Save back to Chemotion
Ideal For
- Chemistry researchers
- University teaching labs
- Collaborative or open science
- Archiving chromatographic results
Author & Repository
- Name: Shilpa Thotli
- Email: shilpa.thotli@kit.edu
The TLC Image Annotation Tool is an open-source project. The source code and further documentation can be found on:
TLC Image Annotation Tool